拟南芥缺乏转录延伸因子 TFIIS 的突变体的种子休眠缺陷是由 DOG1 基因表达减少引起的。
The seed dormancy defect of Arabidopsis mutants lacking the transcript elongation factor TFIIS is caused by reduced expression of the DOG1 gene.
机构信息
Cell Biology & Plant Biochemistry, Biochemie-Zentrum Regensburg, Regensburg University, Universitätsstr. 31, D-93053 Regensburg, Germany; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark.
Cell Biology & Plant Biochemistry, Biochemie-Zentrum Regensburg, Regensburg University, Universitätsstr. 31, D-93053 Regensburg, Germany; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark.
出版信息
FEBS Lett. 2014 Jan 3;588(1):47-51. doi: 10.1016/j.febslet.2013.10.047. Epub 2013 Nov 16.
TFIIS is a transcript elongation factor that facilitates transcription by RNA polymerase II, as it assists the enzyme to bypass blocks to mRNA synthesis. Previously, we have reported that Arabidopsis plants lacking TFIIS exhibit reduced seed dormancy. Among the genes differentially expressed in tfIIs seeds, the DOG1 gene was identified that is a known QTL for seed dormancy. Here we have analysed plants that overexpress TFIIS in wild type background, or that harbour an additional copy of DOG1 in tfIIs mutant background. These experiments demonstrate that the down-regulation of DOG1 expression causes the seed dormancy phenotype of tfIIs mutants.
TFIIS 是一种转录延伸因子，可通过 RNA 聚合酶 II 促进转录，因为它有助于该酶绕过 mRNA 合成的障碍。以前，我们曾报道过缺乏 TFIIS 的拟南芥植物表现出降低的种子休眠。在 tfIIs 种子中差异表达的基因中，鉴定出 DOG1 基因是种子休眠的已知 QTL。在这里，我们分析了在野生型背景下过表达 TFIIS 的植物，或在 tfIIs 突变体背景下含有额外 DOG1 拷贝的植物。这些实验表明，下调 DOG1 的表达导致 tfIIs 突变体的种子休眠表型。
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