几丁寡糖的生产从 Rhizopus oligosporus NRRL2710 细胞壳聚糖酶消化。
Production of chitooligosaccharides from Rhizopus oligosporus NRRL2710 cells by chitosanase digestion.
机构信息
University of Andalas, Padang, West Sumatera, Indonesia.
Department of Advanced Bioscience, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan.
出版信息
Carbohydr Res. 2014 Jan 13;383:27-33. doi: 10.1016/j.carres.2013.06.002. Epub 2013 Jun 14.
The intact cells of Rhizopus oligosporus NRRL2710, whose cell walls are abundant source of N-acetylglucosamine (GlcNAc) and glucosamine (GlcN), were digested with three chitinolytic enzymes, a GH-46 chitosanase from Streptomyces sp. N174 (CsnN174), a chitinase from Pyrococcus furiosus, and a chitinase from Trichoderma viride, respectively. Solubilization of the intact cells by CsnN174 was found to be the most efficient from solid state CP/MAS (13)C NMR spectroscopy. Chitosanase products from Rhizopus cells were purified by cation exchange chromatography on CM-Sephadex C-25 and gel-filtration on Cellulofine Gcl-25m. NMR and MALDI-TOF-MS analyses of the purified products revealed that GlcN-GlcNAc, (GlcN)2-GlcNAc, and (GlcN)2 were produced by the enzymatic digestion of the intact cells. The chitosanase digestion of Rhizopus cells was found to be an excellent system for the conversion of fungal biomass without any environmental impact.
完整的少根根霉 NRRL2710 细胞，其细胞壁是 N-乙酰葡萄糖胺（GlcNAc）和氨基葡萄糖（GlcN）的丰富来源，分别用三种几丁质酶进行消化，一种来自链霉菌 N174 的 GH-46 壳聚糖酶（CsnN174）、一种来自 Pyrococcus furiosus 的几丁质酶和一种来自绿色木霉的几丁质酶。通过固态 CP/MAS（13）C NMR 光谱发现，CsnN174 对完整细胞的溶解效率最高。从 Rhizopus 细胞中纯化的壳聚糖酶产物通过 CM-Sephadex C-25 上的阳离子交换层析和 Cellulofine Gcl-25m 上的凝胶过滤进行纯化。对纯化产物的 NMR 和 MALDI-TOF-MS 分析表明，GlcN-GlcNAc、（GlcN）2-GlcNAc 和（GlcN）2 是通过完整细胞的酶促消化产生的。发现 Rhizopus 细胞的壳聚糖酶消化是一种在没有任何环境影响的情况下转化真菌生物质的极好系统。
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