Institut für Biologie II, Universität Tübingen, Lehrstuhlfür Genetik, Auf der Morgenstelle 28, D-7400, Tübingen, Federal Republic of Germany.
Theor Appl Genet. 1985 Jun;70(3):300-5. doi: 10.1007/BF00304915.
In flower extracts of defined genotypes of Petunia hybrida, an enzyme activity was demonstrated which catalyses the hydroxylation of naringenin and dihydrokaempferol in the 3'-position. Similar to the flavonoid 3'-hydroxylases of other plants, the enzyme activity was found to be localized in the microsomal fraction and the reaction required NADPH as cofactor. A strict correlation was found between 3'-hydroxylase activity and the gene Ht1, which is known to be involved in the hydroxylation of flavonoids in the 3'-position in Petunia. Thus, the introduction of the 3'-hydroxyl group is clearly achieved by hydroxylation of C15-intermediates, and the concomitant occurrence of the 3',4'-hydroxylated flavonoids quercetin and cyanidin (paeonidin) in the presence of the functional allele Ht1 is due to the action of one specific hydroxylase catalysing the hydroxylation of common precursors for both flavonols and anthocyanins.
在已确定基因型的矮牵牛属植物花提取物中，证明了一种酶活性，它能催化柚皮素和二氢山柰酚在 3'位的羟化。与其他植物的类黄酮 3'-羟化酶相似，该酶活性定位于微粒体部分，反应需要 NADPH 作为辅助因子。发现 3'-羟化酶活性与基因 Ht1 之间存在严格的相关性，已知该基因参与矮牵牛属植物中黄酮类化合物在 3'位的羟化。因此，引入 3'-羟基基团显然是通过 C15 中间体的羟化来实现的，并且在功能等位基因 Ht1 的存在下，3',4'-羟基化的类黄酮槲皮素和矢车菊素（芍药素）的同时出现是由于一种特定的羟化酶催化共同前体的羟化，这些前体既是类黄酮醇又是花色素苷。
