Galliano H, Müller A E, Lucht J M, Meyer P
Max Delbrück Laboratorium, Köln, Germany.
Mol Gen Genet. 1995 Jun 10;247(5):614-22. doi: 10.1007/BF00290353.
The transformation booster sequence (TBS) from Petunia hybrida enhances transformation frequencies in P. hybrida, Nicotiana tabacum and Zea mays. TBS also stimulates homologous inter- and intramolecular recombination in P. hybrida, the molecular basis for this stimulation is not known. We investigated whether TBS contains sequence elements that might contribute to the stimulation of recombination and whether its recombinogenic potential reflects a biological function of TBS. We identified a scaffold attachment region (SAR) within TBS and analysed its distribution in the genome and its homologies to other genomic sequences. A 516 bp subfragment of TBS binds to the nuclear scaffold. The sequence of the TBS-SAR fragment shows strong homologies to retroviral elements from plants, suggesting that TBS is an inactive derivative of a retrovirus that still promotes DNA recombination.
矮牵牛的转化增强序列（TBS）可提高矮牵牛、烟草和玉米的转化频率。TBS还能刺激矮牵牛中的同源分子间和分子内重组，但其刺激的分子基础尚不清楚。我们研究了TBS是否包含可能有助于刺激重组的序列元件，以及其重组潜能是否反映了TBS的生物学功能。我们在TBS中鉴定出一个支架附着区域（SAR），并分析了其在基因组中的分布及其与其他基因组序列的同源性。TBS的一个516 bp亚片段与核支架结合。TBS-SAR片段的序列与植物逆转录病毒元件具有很强的同源性，这表明TBS是一种逆转录病毒的无活性衍生物，仍能促进DNA重组。
