Tissue Culture Laboratories, Punjab Agricultural University, 141 004, Ludhiana, India.
Theor Appl Genet. 1985 Jul;70(4):363-8. doi: 10.1007/BF00273740.
Through the in vitro culture of excised embryos and ovules, interspecific hybrids have been obtained from cultivated and wild species of Gossypium. The hybrids matured upon transfer to the field. The anthers, ovules and embryos from both the diploid (2n=26) and tetraploid (2n=52) species underwent proliferation, and this response was genotypic. The diploid species invariably showed profuse callusing in comparison with the tetraploid. The callus showed various chromosome numbers, ranging from haploids to hexaploids, and from high polyploidy to aneuploidy. Hybrid callus culture may augment the genetic variability by providing a means for obtaining genetic exchange in interspecific hybrids. The implications of the in vitro induction of genetic variability for cotton improvement are discussed.
通过离体培养胚和胚珠，已经从栽培和野生的棉属物种中获得了种间杂种。杂种在转移到田间后成熟。来自二倍体（2n=26）和四倍体（2n=52）物种的花药、胚珠和胚胎都发生了增殖，这种反应是基因型的。与四倍体相比，二倍体总是表现出大量愈伤组织。愈伤组织表现出各种染色体数目，从单倍体到六倍体，从高多倍体到非整倍体。杂种愈伤组织培养可以通过提供种间杂种遗传交换的手段来增加遗传变异性。讨论了体外诱导遗传变异对棉花改良的意义。
