D-[3H]aspartate retrograde labelling of association neurones in area SI of the cat.

Injection of D-[3H]aspartate into area SII of cats retrogradely labelled association cells in area SI. Numerous intensely labelled cells were found in layer II and in the upper layer III but labelling was scanty in other layers. In contrast, association neurones labelled by horseradish peroxidase injected in area SII mixed with the radioactive marker were also numerous in the other sublaminae of layer III and in infragranular layers of SI. Association neurones of the outer laminae of this area are likely to use aspartate and/or glutamate as neurotransmitter(s).