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利用 Ri T-DNA 对马铃薯发育进行遗传修饰。

Genetic modification of potato development using Ri T-DNA.

机构信息

Department of Biochemistry, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., England.

出版信息

Theor Appl Genet. 1985 Jul;70(4):440-6. doi: 10.1007/BF00273752.

DOI:10.1007/BF00273752
PMID:24253018
Abstract

Forty-two potato plants were regenerated from a hairy-root line obtained after infection of a shoot of Solanum tuberosum cv 'Desiree' with Agrobacterium rhizogenes strain LBA 9402 (pRil855). Transformed plants were uniform and had a distinct phenotype and development compared with untransformed controls. Their growth was vigorous, especially early in their development, their roots were abundant and showed reduced geotropism, their leaves were slightly crinkled and glossy and they produced longer tubers with more frequent, prominent eyes. Cytological examination showed that ten of the forty-two transformed plants had either 47 or 49 chromosomes instead of the normal 48. In two of these aneuploids structural changes were observed.

摘要

从感染了根癌农杆菌 LBA 9402(pRil855)菌株的马铃薯 cv 'Desiree' 茎段中获得的发根系再生了 42 株马铃薯植株。与未转化对照相比,转化植株具有均匀的、明显的表型和发育特征。它们的生长非常旺盛,尤其是在早期发育阶段,根系丰富,向地性减弱,叶片稍有皱缩且有光泽,产生的块茎更长,眼芽更频繁、更突出。细胞学检查表明,在这 42 株转化植株中有 10 株的染色体要么是 47 条,要么是 49 条,而不是正常的 48 条。在其中两个非整倍体中观察到了结构变化。

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本文引用的文献

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T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato.利用 T-DNA 基因研究植物发育:根癌农杆菌转化马铃薯中超早花和细胞分裂素的增强。
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9
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From tumour to tuber; tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (Solanum tuberosum cv. 'Maris Bard').从肿瘤到结核;冠瘿瘤衍生的四倍体马铃薯植株(Solanum tuberosum cv. 'Maris Bard')的肿瘤细胞特征和染色体数目。
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