Desmet Siel, Dhooghe Emmy, De Keyser Ellen, Quataert Paul, Eeckhaut Tom, Van Huylenbroeck Johan, Geelen Danny
Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food Research, Melle, Belgium.
Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Front Plant Sci. 2020 Jun 23;11:859. doi: 10.3389/fpls.2020.00859. eCollection 2020.
infects and transforms a wide range of plant species. It thereby introduces new genes located on transfer-DNA of the root inducing plasmid (pRi) into the plant genome and one of its abilities is to alter the host root system. Explants from pRi transformed roots from were regenerated to create naturally transgenic Ri lines. The presence of and genes in the Ri lines was linked with altered growth characteristics: shorter peduncles, wrinkled leaves, delayed flowering and enhanced root growth. The potential of Ri lines for breeding was evaluated through consecutive backcrossing with the original host genotype. The progeny of reciprocal crosses showed non-Mendelian inheritance suggesting partial transmission of the of the and genes. The typical Ri phenotype observed in the primary Ri line was partially inherited. These results revealed that the Ri phenotype is a complex trait influenced by the genetic background of the Ri line.
感染并转化多种植物物种。它由此将位于发根诱导质粒（pRi）的转移DNA上的新基因导入植物基因组，其能力之一是改变宿主根系。来自pRi转化根的外植体被再生以创建天然转基因Ri系。Ri系中 和 基因的存在与改变的生长特性相关：较短的花柄、皱叶、延迟开花和增强的根系生长。通过与原始宿主基因型连续回交来评估Ri系的育种潜力。正反交的后代显示出非孟德尔遗传，表明 和 基因的 部分传递。在初级Ri系中观察到的典型Ri表型部分遗传。这些结果表明，Ri表型是一个受Ri系遗传背景影响的复杂性状。
