National Food Research Institute, Tsukuba Science City, P.O. Box 11, 305, Ibaraki, Japan.
Plant Cell Rep. 1984 Feb;3(1):23-6. doi: 10.1007/BF00270223.
The regulation of starch synthesis and exocellular polysaccharide synthesis by GA3 was studied with cells of sweet potato grown as suspension in glycerol medium. In the presence of GA3, and under normal cell growth, starch formation was inhibited. The incorporation activity (starch synthesis) from ADP-[(14)C] glucose or UDP-[(14)C] glucose with GA3 treated cells was reduced. On the other hand, the synthesis of exocellular polysaccharides composed of glucose, galactose, mannose and arabinose etc., was stimulated and a clear increase of the Man/Ara ratio was observed in the presence of GA3. These results may indicate that GA3 affects the regulation of starch synthesis and exocellular polysaccharide synthesis.
用甘油脂培养基悬浮培养的甘薯细胞作为材料，研究了 GA3 对淀粉合成和细胞外多糖合成的调控作用。在 GA3 存在的情况下，在正常细胞生长的条件下，淀粉的形成受到抑制。用 GA3 处理的细胞中 ADP-[(14)C]葡萄糖或 UDP-[(14)C]葡萄糖的掺入活性（淀粉合成）降低。另一方面，由葡萄糖、半乳糖、甘露糖和阿拉伯糖等组成的细胞外多糖的合成受到刺激，并且在 GA3 的存在下观察到 Man/Ara 比的明显增加。这些结果可能表明 GA3 影响淀粉合成和细胞外多糖合成的调控。
