芸薹属种体细胞遗传研究：I. 白菜型油菜（L.）H. f. & T. 中高频产生单倍体植株。

Somatic cell genetic studies inBrassica species : I. High frequency production of haploid plants inBrassica alba (L.) H. f. & T.

机构信息

Programme in Genetical Research, Department of Botany, Bose Institute, 700 009, Calcutta, India.

出版信息

Plant Cell Rep. 1984 Jun;3(3):102-5. doi: 10.1007/BF02441010.

Abstract

In vitro culture ofBrassica alba anthers on a growth medium containing inorganics of KB5 and organics, iron, sucrose and hormones of B5 resulted in a very high response of anthers (93.75%) towards callus induction. All the calli transferred to regeneration media responded favourably even after six months of callus induction. Numerous torpedo-shaped embryoids developed in clusters at many sites from each callus mass. Secondary embryogenesis and multiple shoot formation was also observed in many cases. The number of embryoids and plantlets produced by one embryogenic anther were as high as 169.8 and 17 respectively. 87% of the regenerated plants were haploids.

摘要

在含有 KB5 无机物和有机物、铁、蔗糖以及 B5 激素的生长培养基上对白菜型油菜花药进行离体培养，花药的愈伤组织诱导率非常高（93.75%）。所有转移到再生培养基上的愈伤组织即使在经过六个月的诱导后也能很好地响应。许多鱼雷形胚状体在每个愈伤组织块的许多部位成簇发育。在许多情况下，还观察到次生胚胎发生和多个芽的形成。一个胚性花药产生的胚状体和小植株的数量高达 169.8 和 17 个。再生植株中有 87%是单倍体。