Tissue Culture Section, Bose Institute Kankurgachi, 700 054, Calcutta, India.
Plant Cell Rep. 1985 Oct;4(5):281-4. doi: 10.1007/BF00269378.
Plantlets were obtained from leaf explants of a Labiatae tree - Leucosceptrum canum Sm. using plant tissue culture techniques. Two types of calli proliferated from the leaf explants when grown on different media, one of which was amenable to somatic embryogenesis. Differentiation of the embryoids started from the fourth passage of culture and continued up to the seventh passage. The number of embryoids decreased with the age of the callus. The capacity of such embryoids to form entire plantlets was studied using different nutrient mileux. Embryoids formed plantlets on Murashige and Skoog's (MS) medium fortified with benzylaminopurine plus indolebutyric acid. Organogenesis was observed in shoot-buds derived from explants of in vitro regenerated plantlets on MS basal medium supplemented with benzylaminopurine. Culture regenerated plantlets were transferred to MS medium without sucrose and growth hormones; finally transferred to pots containing sterile vermiculite where they are growing.
采用植物组织培养技术，从唇形科树 - Leucosceptrum canum Sm. 的叶片外植体中获得小植株。当在不同的培养基上生长时，两种类型的愈伤组织从叶片外植体中增殖，其中一种愈伤组织适合体细胞胚胎发生。胚状体的分化从第四代培养开始，并持续到第七代。胚状体的数量随愈伤组织的年龄而减少。通过不同的营养环境研究了这种胚状体形成完整小植株的能力。胚状体在添加苄氨基嘌呤和吲哚丁酸的 Murashige 和 Skoog（MS）培养基上形成小植株。在补充有苄氨基嘌呤的 MS 基本培养基上，从体外再生小植株的外植体衍生的芽中观察到器官发生。将培养再生的小植株转移到不含蔗糖和生长激素的 MS 培养基中；最后转移到含有无菌蛭石的盆中，在那里生长。
