Department of Pharmacy, University of Manchester, M13 9PL, Manchester, UK.
Plant Cell Rep. 1984 Jun;3(3):109-11. doi: 10.1007/BF02441012.
Cell cultures ofNicotiana tabacum were grown on M & S medium containing 0-2.0 ppm of two auxins. Cellular nicotine was estimated for given auxin concentrations and highest levels determined. Feeding suspensions with amino acids either alone, or in combination with nicotinic acid and nicotinamide produced decresed nicotine levels. The presence of anatabine, anabasine, myosmine, anatalline and nicotelline in the cultures was established and it was noted that precursor feeding which decreased nicotine concentration caused elevated levels of the other alkaloids, notably anatabine.
将烟草细胞培养在含有 0-2.0 ppm 两种植物生长素的 M & S 培养基上。为特定的生长素浓度估计细胞内尼古丁，并确定最高水平。用氨基酸单独或与烟酸和烟酰胺一起喂养悬浮液会降低尼古丁水平。在培养物中发现了 anatabine、anabasine、myosmine、anatalline 和 nicotelline，并注意到降低尼古丁浓度的前体喂养导致其他生物碱，特别是 anatabine 的水平升高。
