Department of Anatomy and Cell Biology, University of Cantabria, 39011, Santander, Spain,
Cell Tissue Res. 2014 Feb;355(2):397-407. doi: 10.1007/s00441-013-1746-z. Epub 2013 Nov 20.
We describe the structure of the lympho-granulocytic tissue associated with the wall of the spiral valve of the African lungfish Protopterus annectens. The study was performed under freshwater conditions and after 6 months of aestivation. The lympho-granulocytic tissue consists of nodes surrounded by reticular tissue. The nodes are formed by an outer and an inner component separated by a thin collagenous layer. The outer component is a reticular-like tissue that contains two types of granulocytes, developing and mature plasma cells and melanomacrophage centres (MMCs). The inner component, the parenchyma, contains a meshwork of trabeculae and vascular sinusoids and shows dark and pale areas. The dark areas contain diffuse lymphoid tissue, with a large number of mitoses and plasma cell clusters. The pale areas contain a small number of macrophages and lymphocytes. Macrophages and sinus endothelial cells are filled with haemosiderin granules and appear to form part of the reticuloendothelial system of the lungfish. The reticular tissue houses granulocytes, plasma cells and MMCs and might serve for the housing and maturation of cells of the white series. After aestivation, the nodes undergo lymphocyte depletion, the suppression of mitosis, granulocyte invasion and the occurrence of cell death. By contrast, few histological changes occur in the reticular tissue. Whereas the nodes appear to be involved in lymphocyte proliferation and plasma cell maturation, the function of the reticular tissue remains obscure.
我们描述了与非洲肺鱼 Protopterus annectens 螺旋瓣膜壁相关的淋巴粒细胞组织的结构。该研究是在淡水条件下和经过 6 个月夏眠后进行的。淋巴粒细胞组织由被网状组织包围的结节组成。这些结节由外和内两部分组成，中间有一层薄的胶原层隔开。外部分是一种类似于网状的组织，其中包含两种类型的粒细胞、发育中的和成熟的浆细胞以及黑色素巨噬细胞中心（MMC）。内部分，即实质，包含小梁和血管窦的网状结构，显示出深色和浅色区域。深色区域含有弥漫性淋巴组织，有大量有丝分裂和浆细胞簇。浅色区域含有少量巨噬细胞和淋巴细胞。巨噬细胞和窦内皮细胞充满含铁血黄素颗粒，似乎形成肺鱼网状内皮系统的一部分。网状组织容纳粒细胞、浆细胞和 MMC，并可能为白细胞系列的细胞提供住所和成熟。夏眠后，结节经历淋巴细胞耗竭、有丝分裂抑制、粒细胞浸润和细胞死亡。相比之下，网状组织几乎没有发生组织学变化。虽然结节似乎参与淋巴细胞增殖和浆细胞成熟，但网状组织的功能仍然不清楚。
