Aplin J D, Campbell S
Placenta. 1985 Nov-Dec;6(6):469-79. doi: 10.1016/s0143-4004(85)80001-1.
Using immunofluorescence we have studied the distribution within and beneath the cytotrophoblast of chorion laeve of five extracellular matrix components: types I, III and IV collagen, fibronectin and laminin. Fibronectin, laminin and types I and IV collagen are located in a network of extracellular matrix which encapsulates cells of the cytotrophoblast multilayer. The results suggest that cytotrophoblast in all layers is active in matrix biosynthesis. The pseudo-basement membrane, which links this intercellular matrix to the underlying reticular layer, contains rich deposits of fibronectin and type III collagen along with more restricted foci of laminin and type IV collagen. The reticular layer contains fibronectin, type III collagen and small amounts of type I collagen, but no laminin or type IV collagen. Type III collagen is also found in the maternal decidua. Extracellular matrix markers may be useful in defining trophoblast differentiation pathways. Possible reasons are discussed for the unusual architecture of extracellular matrix of cytotrophoblast of the chorion laeve. Cytoplasmic actin occurs in cells throughout the chorion laeve, while cytokeratin appears only in cytotrophoblast. Plasminogen also occurs throughout cytotrophoblast in pericellular locations.
我们运用免疫荧光技术研究了平滑绒毛膜细胞滋养层内及下方五种细胞外基质成分的分布情况，这五种成分分别是：I型、III型和IV型胶原蛋白、纤连蛋白和层粘连蛋白。纤连蛋白、层粘连蛋白以及I型和IV型胶原蛋白位于细胞外基质网络中，该网络包裹着多层细胞滋养层的细胞。结果表明，所有层的细胞滋养层在基质生物合成方面都很活跃。将这种细胞间基质与下方网状层相连的假基底膜，含有丰富的纤连蛋白和III型胶原蛋白沉积物，同时还有更局限的层粘连蛋白和IV型胶原蛋白聚集区。网状层含有纤连蛋白、III型胶原蛋白和少量I型胶原蛋白，但没有层粘连蛋白或IV型胶原蛋白。III型胶原蛋白在母体蜕膜中也有发现。细胞外基质标志物可能有助于确定滋养层的分化途径。文中讨论了平滑绒毛膜细胞滋养层细胞外基质结构异常的可能原因。整个平滑绒毛膜的细胞中都存在细胞质肌动蛋白，而细胞角蛋白仅出现在细胞滋养层中。纤溶酶原也在整个细胞滋养层的细胞周围位置出现。
