通过连续处理吖啶酮和秋水仙素诱导矮牵牛悬浮培养物中的高有丝分裂指数。
Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine.
机构信息
Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, 50 250, Bet Dagan, Israel.
出版信息
Plant Cell Rep. 1984 Dec;3(6):219-21. doi: 10.1007/BF00269296.
Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml aphidicolin for 18 h, then cultured in drug-free medium for 8 h and finally exposed to 0.1% colchicine for 8 additional hours exhibited MI of 62.8% and 65.7% respectively, for the two cell lines in study.
长春花悬浮细胞经秋水仙素、阿霉素、剧烈换液或阿霉素和秋水仙素顺序处理后，有丝分裂指数（MI）明显高于正常水平。最后一种处理方法产生的 MI 值最高：用 30μg/ml 阿霉素处理 18 小时，然后在无药培养基中培养 8 小时，最后再用 0.1%秋水仙素处理 8 小时，两种实验细胞系的 MI 值分别为 62.8%和 65.7%。
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