Biochemistry Division, National Chemical Laboratory, 411 008, Poona, India.
Plant Cell Rep. 1984 Dec;3(6):222-5. doi: 10.1007/BF00269297.
Complete plants were grown from zygotic embryos cultured on Y3 basal liquid medium supplemented with coconut milk, BA and NAA. Explants from stem, leaf and rachilla of mature coconut trees turned green and swelled on Y3 semi-solid basal media supplemented with 2,4-D, K, NAA, BA and activated charcoal. Callus was initiated in explants from the subapical regions of the stem on Y3 basal medium supplemented with 2,4-D (4.52×10(2)μM). Globular embryo-like structures were obtained when this callus was subcultured to auxinless medium. Root formation was obtained from leaf explants on Y3 basal medium containing citric acid, ascorbic acid and 2,4-D (4.52×10(2) μM). Globular embryo-like structures were also obtained directly from leaf explants on a Y3 basal medium supplemented with 2,4-D (2.26×10(2) μM). Callus isolated from rachilla explants on Y3 basal medium containing 2,4-D(4.52×10(2) μM), formed nodular structures when transferred to medium with 2,4-D (2.3×10(1) μM). These nodules developed roots from the base of the nodular growth whereas from the upper portion shoots were observed on Y3 basal liquid medium.
完整的植株是由在添加了椰子奶、BA 和 NAA 的 Y3 基础液体培养基上培养的合子胚生长而成的。成熟椰子树的茎、叶和穗轴外植体在添加了 2,4-D、K、NAA、BA 和活性炭的 Y3 半固体基础培养基上变绿并膨胀。在添加了 2,4-D（4.52×10(2) μM）的 Y3 基础培养基上，从茎的亚顶端区域的外植体中启动愈伤组织。当将这种愈伤组织继代到不含生长素的培养基中时，会获得球形胚样结构。从含有柠檬酸、抗坏血酸和 2,4-D（4.52×10(2) μM）的 Y3 基础培养基上的叶片外植体获得生根。也可以直接从添加了 2,4-D（2.26×10(2) μM）的 Y3 基础培养基上的叶片外植体获得球形胚样结构。从含有 2,4-D（4.52×10(2) μM）的 Y3 基础培养基上的穗轴外植体分离的愈伤组织，当转移到含有 2,4-D（2.3×10(1) μM）的培养基中时，会形成结节状结构。这些结节从结节生长的底部发育出根，而在 Y3 基础液体培养基的上部观察到芽。
