Fisher J B, Tsai J H
In Vitro. 1978 Mar;14(3):307-11. doi: 10.1007/BF02616041.
A medium for optimal growth of embryos of Jamaican Tall and Green Malayan Dwarf varieties of coconut palm was developed. The Liquid basal Murashige and Skoog medium was supplemented with coconut milk, IAA and 2IP. Activated charcoal improved embryo growth on agar medium. A single callus line was initiated from solid endosperm and subcultured on basal Schenk and Hildebrandt medium supplemented with 2 mg per 1 NAA. Attempts at inducing organogenesis in the callus were unsuccessful. No vascular tissue was present. The callus was aneuploid with the chromosome number = 8 (normal 2n = 32).
开发了一种用于牙买加高种和绿色马来矮种椰子胚胎最佳生长的培养基。液体基础Murashige和Skoog培养基添加了椰乳、吲哚乙酸（IAA）和异戊烯腺嘌呤（2IP）。活性炭可促进胚胎在琼脂培养基上的生长。从固体胚乳中诱导出一条愈伤组织系，并在添加每升2毫克萘乙酸（NAA）的基础Schenk和Hildebrandt培养基上进行继代培养。在愈伤组织中诱导器官发生的尝试未成功。不存在维管组织。该愈伤组织为非整倍体，染色体数 = 8（正常2n = 32）。
