Botanisches Institut der Universität Köln, Gyrhofstrasse 15, D-5000, Köln 41, Federal Republic of Germany.
Planta. 1984 May;161(2):142-7. doi: 10.1007/BF00395474.
Seedlings of red radish (Raphanus sativus L. var. sativus) accumulated high amounts of free malic acid and sinapoylmalate, when grown on nitrate as the sole N-source. In the presence of ammonium (NO 3 (-) : NH 4 (+) , 1:2) both metabolites failed to accumulate, and the levels of arginine, asparagine, glutamine, histidine, and serine were greatly increased. The extractable activity of 1-sinapoylglucose: L-malate sinapoyltransferase, an enzyme which plays a key role in channelling malic acid into the sinapic-acid metabolism of this plant, was positively correlated with the malic-acid level in cotyledons. The possibility is discussed that free malic acid might be the likely candidate for regulating the activity of 1-sinapoylglucose: L-malate sinapoyltransferase.
萝卜（Raphanus sativus L. var. sativus）幼苗在以硝酸盐作为唯一氮源的情况下，会积累大量的游离苹果酸和芥子酰苹果酸。当存在铵（NO3-：NH4+，1:2）时，这两种代谢物都无法积累，而精氨酸、天冬酰胺、谷氨酰胺、组氨酸和丝氨酸的水平则大大增加。1-芥子酰葡萄糖：L-苹果酸芥子酰转移酶的可提取活性，这种酶在将苹果酸定向到植物的芥子酸代谢中起着关键作用，与子叶中的苹果酸水平呈正相关。人们讨论了游离苹果酸可能是调节 1-芥子酰葡萄糖：L-苹果酸芥子酰转移酶活性的候选物质。
