玉米根系中有机酸的区室化 I. 两种苹果酸池的差异标记。
Compartmentation of organic acids in corn roots I. Differential labeling of 2 malate pools.
机构信息
Department of Biological Sciences, Purdue University, Lafayette, Indiana.
出版信息
Plant Physiol. 1966 Apr;41(4):709-12. doi: 10.1104/pp.41.4.709.
Bicarbonate-(14)C and acetate-(3)H were simultaneously provided to corn roots to give 2 isotopic forms of malate in the tissue, malate-(14)C produced by dark fixation reactions and malate-(3)H produced by reactions of the tricarboxylic acid cycle. Following a short pulse of exposure to the isotopes, the dissimilation of both isotopic forms of malic acid was followed. The rate of utilization of malate-(3)H was much faster than that of malate-(14)C.These results are interpreted as showing that the malate produced from (14)CO(2) is in a pool physically separated from that in the tricarboxylic acid cycle. The introduction of the 2 isotopes through distinct metabolic pathways produced the differential labeling of 2 distinct pools of malate.
碳酸氢盐-(14)C 和醋酸盐-(3)H 同时提供给玉米根，在组织中产生 2 种同位素形式的苹果酸，苹果酸-(14)C 通过暗固定反应产生，苹果酸-(3)H 通过三羧酸循环的反应产生。在短暂暴露于同位素后，跟踪两种同位素形式的苹果酸的异化。苹果酸-(3)H 的利用率远高于苹果酸-(14)C。这些结果表明，由 (14)CO(2) 产生的苹果酸位于与三羧酸循环中不同的物理分隔的池中。通过不同的代谢途径引入这 2 种同位素，产生了 2 种不同的苹果酸池的差异标记。
Zotero 插件