Botanisches Institut der Universität, Schlossgarten 4, D-8520, Erlangen, Federal Republic of Germany.
Planta. 1984 May;161(3):216-22. doi: 10.1007/BF00982915.
In the green algaMougeotia, the dichroic orientation of the red-absorbing form of phytochrome (Pr) is parallel of the cell surface, whereas the far-red-absorbing form (Pfr) is oriented normal to it. The time course of the change from parallel to normal was investigated by double-flash irradiation with polarized red and far-red light. The results obtained by two different methods indicate that most of the phytochrome intermediates existing in the first 5 ms after the inducing red flash are still oriented parallel to the cell surface, similar to Pr. At increasing intervals between the red and the far-red flashes, more and more phytochrome molecules turn their transition moments to the Pfr orientation. This reaction is finished after approximately 30 ms. We conclude that the change in dichroic orientation of the phytochrome molecules inMougeotia occurs during the last relaxation steps of the intermediates on the way from Pr to Pfr. It cannot be decided yet, whether the first surface-normal phytochrome species is an intermediate or Pfr itself.
在绿藻衣藻中，红光吸收型的光敏色素（Pr）的二向色性取向与细胞表面平行，而远红光吸收型（Pfr）则与之垂直。通过用偏振红光和远红光进行双闪光照射，研究了从平行到垂直的变化的时间过程。通过两种不同方法获得的结果表明，在诱导红光闪光后的最初 5 毫秒内存在的大多数光敏色素中间物仍然与 Pr 相似，平行于细胞表面取向。随着红光和远红光闪光之间的间隔增加，越来越多的光敏色素分子将其跃迁矩转向 Pfr 取向。该反应在大约 30 毫秒后完成。我们的结论是，在从 Pr 到 Pfr 的中间过程的最后弛豫步骤中，衣藻中光敏色素分子的二向色性取向的变化发生。目前尚不能确定第一个表面垂直的光敏色素物种是中间物还是 Pfr 本身。
