Laboratory of Plant Breeding, Faculty of Agriculture, Kyushu University, Hakozaki, 812, Fukuoka, Japan.
Theor Appl Genet. 1985 May;69(3):253-7. doi: 10.1007/BF00662436.
Five mutant lines of rice with increased amylose content in starch granules were identified among floury endosperm mutants. The amylose contents of the mutants ranged from 29.4% to 35.4% and were about twice as high as that of the normal counterpart. Starch properties of the high amylose mutants were analyzed by column chromatography, X-ray diffractometry, photopastegraphy and scanning electron microscopy. The high amylose mutants produced longer unit chains of amylopectin than those of the normal counterpart as well as an increased amount of amylose. A X-ray diffractogram of starch in the mutant was characterized by a type B pattern, while that in the normal counterpart showed a type A pattern which is typical for starches of common cereals. The temperatures at the initiation of gelatinization of the mutants were much higher than that for the normal counterpart. The endosperm cells of the mutant were loosely packed with irregular round-shaped starch granules, whereas those of the normal counterpart were densely packed with polyhedral starch granules. Judging from the results obtained, it was concluded that starch properties of the high amylose mutants of rice were similar to those of the amylose-extender (ae) mutant of maize.
在粉质胚乳突变体中鉴定出了 5 个淀粉粒直链淀粉含量增加的突变系。突变体的直链淀粉含量在 29.4%至 35.4%之间，约为正常对照的两倍。通过柱层析、X 射线衍射、光镜和扫描电子显微镜分析了高直链淀粉突变体的淀粉性质。高直链淀粉突变体产生的支链淀粉长链单位比正常对照多，直链淀粉含量也增加。突变体淀粉的 X 射线衍射图谱具有 B 型图案，而正常对照则呈现出典型的普通谷物淀粉的 A 型图案。突变体淀粉的起始胶化温度远高于正常对照。突变体的胚乳细胞松散排列，呈不规则圆形淀粉粒，而正常对照则紧密排列多面体形淀粉粒。从获得的结果可以得出结论，水稻高直链淀粉突变体的淀粉性质与玉米直链淀粉扩展（ae）突变体相似。
