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普通氨基酸渗透酶可诱导小球藻中表达。

A general amino-acid permease is inducible in Chlorella vulgaris.

机构信息

Institut für Botanik der Universität, Universitätsstrasse 31, D-8400, Regensburg, Federal Republic of Germany.

出版信息

Planta. 1984 Jul;161(5):425-31. doi: 10.1007/BF00394573.

Abstract

Glucose or non-metabolizable glucose analogues induce two systems of amino-acid transport in Chlorella vulgaris: an arginine-lysine system and a proline system. An additional third system of amino-acid transport is induced when glucose and an inorganic nitrogen source are present during glucose induction. The transport rates in glucose-NH 4 (+) -treated cells are 10 to 80 times higher than in untreated cells. The transport system shows a rather broad specificity and catalyses the transport of at least ten neutral and acidic amino acids. Three of these amino acids (L-alanine, L-serine and glycine) are transported by the proline system as well. The system is specific for L-amino acids and has a pH optimum between 5 and 6. Transport by this system seems to be active, since amino acids are accumulated inside the cells.

摘要

葡萄糖或非代谢性葡萄糖类似物诱导小球藻中的两种氨基酸运输系统

精氨酸-赖氨酸系统和脯氨酸系统。当葡萄糖诱导过程中存在葡萄糖和无机氮源时,会诱导第三种氨基酸运输系统。在葡萄糖-NH 4 (+) 处理的细胞中,氨基酸的转运速率比未处理的细胞高 10 到 80 倍。该转运系统具有较广泛的特异性,可催化至少十种中性和酸性氨基酸的转运。其中三种氨基酸(L-丙氨酸、L-丝氨酸和甘氨酸)也可通过脯氨酸系统转运。该系统对 L-氨基酸具有特异性,最适 pH 值在 5 到 6 之间。由于氨基酸在细胞内积累,因此通过该系统的转运似乎是主动的。

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