Forestry Sciences Laboratory, Rocky Mountain Forest and Range Experiment Station, 86001, Flagstaff, Arizona.
J Chem Ecol. 1992 Apr;18(4):543-60. doi: 10.1007/BF00987818.
We have developed and tested a technique to microencapsulate terpene compounds by forming gelatin-walled microcapsules around the terpene essential oils. Eight terpenes that are common constituents of Douglas fir (Pseudotsuga menziesii) oleoresin were encapsulated, including five monoterpenes (α-pinene,β-pinene, camphene, myrcene, and limonene) and three oxygenated monoterpenes (bornyl acetate, linalool, andβ-citronellol). The encapsulated terpenes were mixed into artificial diets to determine the effects they had on western spruce budworm (Choristoneura occidentalis) survival and reproduction, using a three-generation bioassay. We describe the technique and present data from preliminary bioassays with seven of the terpenes to demonstrate thatC. occidentalis larvae would consume diets fortified with microencapsulated terpenes. The technique is useful because it helps to reduce the rate at which terpenes volatilize from the diets and it "packages" the terpenes in a manner more closely resembling the way they are present in the resin canals of plant foliage. Many scientists investigating the biological importance of terpenes in mediating plant-herbivore interactions may find that microencapsulated terpenes are useful in both laboratory diet studies and field and greenhouse experiments with plant material.
我们开发并测试了一种通过在萜烯精油周围形成明胶壁微胶囊来微囊化萜类化合物的技术。八种萜类化合物是道格拉斯冷杉（Pseudotsuga menziesii）松脂的常见成分，包括五种单萜类化合物（α-蒎烯、β-蒎烯、莰烯、月桂烯和柠檬烯）和三种含氧单萜类化合物（乙酸龙脑酯、芳樟醇和β-香茅醇）。将封装的萜类化合物混合到人工饲料中，使用三代生物测定法确定它们对西部云杉芽虫（Choristoneura occidentalis）生存和繁殖的影响。我们描述了该技术，并提供了用七种萜类化合物进行初步生物测定的数据，以证明 C.occidentalis 幼虫会消耗强化了微囊化萜类化合物的饲料。该技术很有用，因为它有助于降低萜类化合物从饲料中挥发的速度，并且以更类似于萜类化合物在植物叶组织的树脂道中存在的方式“封装”它们。许多研究萜类化合物在介导植物-食草动物相互作用中的生物学重要性的科学家可能会发现，微囊化萜类化合物在实验室饮食研究以及与植物材料的田间和温室实验中都很有用。
