从成熟的花旗松（Pseudotsuga menziesii）和糖松（Pinus lambertiana）母树上进行 Shoot 增殖。
Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana).
机构信息
Department of Pomology, University of California, 95616, Davis, CA, USA.
出版信息
Plant Cell Rep. 1985 Aug;4(4):177-9. doi: 10.1007/BF00269282.
Opening of apical and axillary buds of mature Douglas-fir and sugar pine trees was obtained on a newly formulated basal medium (DCR) without growth regulators. Elongation of buds was observed on 1/2 strength DCR with 0.3% activated charcoal (DCR-1). In sugar pine, multiple shoots were obtained when explants on DCR with 0.5 mg/1 BAP for 5-6 weeks were transferred to DCR-1 medium. On subculture, axillary buds again developed when shoots were cultured on DCR with 0.2 mg/1 BAP for Douglas-fir and 0.5 mg/1 BAP for sugar pine. These buds were again elongated on DCR-1 medium. By subculturing 7-10 shoots of Douglas-fir and 2-3 shoots of sugar pine, over 100 shoots can be obtained in a year.
在新配方的基础培养基（DCR）上，无需生长调节剂即可获得成熟的花旗松和糖松顶芽和腋芽的开放。在 1/2 强度的 DCR 中添加 0.3%活性炭（DCR-1）可观察到芽的伸长。在糖松中，当将在含有 0.5 mg/1 BAP 的 DCR 上培养 5-6 周的外植体转移到 DCR-1 培养基上时，可获得多个芽。在继代培养中，当将芽在含有 0.2 mg/1 BAP 的 DCR 上培养时，腋芽再次发育为花旗松，而在含有 0.5 mg/1 BAP 的 DCR 上培养时则为糖松。这些芽再次在 DCR-1 培养基上伸长。通过继代培养 7-10 个花旗松芽和 2-3 个糖松芽，一年可获得超过 100 个芽。
Zotero 插件