苜蓿和黄花苜蓿的核酮糖-1,5-二磷酸羧化酶-加氧酶碱性大亚基的异质性。

Heterogeneity of alkaline small subunits of ribulose 1,5 bisphosphate carboxylase-oxygenase from Medicago sativa and M. falcata.

机构信息

Division of Plant Industry, CSIRO, P.O. Box 1600, 2601, Canberra City, ACT, Australia.

出版信息

Plant Cell Rep. 1985 Aug;4(4):212-5. doi: 10.1007/BF00269292.

Abstract

The isolated leaf proteins of lucerne (Medicago sativa L. and M. falcata L.) were fractionated by Sepharose 6B column chromatography. Analysis of fractionated proteins indicated that the 2nd peak component was almost entirely ribulose 1,5 bisphosphate carboxylase-oxygenase (Rubisco) which represented 57% of the total recovered protein.Rubisco yielded one large subunit (LSU) and one small subunit (SSU) polypeptide after SDS gel electrophoresis.Isoelectric focusing of the SSU of Rubisco from genotypes of M. sativa cv. Hunter River (HR), Hairy Peruvian (HP) and of M. falcata (MF) showed two SSU components for HR and HP, and three components for MF. Most components of genotypes were located in the alkaline region of the gel. While the pIs of the SSU components of HR and HP were identical they differed from those of the SSU of MF thus demonstrating heterogeneity for SSU in Medicago.It is suggested that the alkaline nature of SSU may have some adaptive physiological significance.

摘要

用葡聚糖凝胶 6B 柱层析法分离了紫花苜蓿（Medicago sativa L. 和 M. falcata L.）的分离叶蛋白。对分级蛋白质的分析表明，第 2 峰组分几乎完全是核酮糖 1,5-二磷酸羧化酶-加氧酶（Rubisco），占回收总蛋白的 57%。Rubisco 在 SDS 凝胶电泳后产生一个大亚基（LSU）和一个小亚基（SSU）多肽。来自紫花苜蓿品种亨特河（HR）、多毛秘鲁（HP）和 M. falcata（MF）的 Rubisco SSU 的等电聚焦显示 HR 和 HP 有两个 SSU 组分，MF 有三个组分。大多数基因型的组分位于凝胶的碱性区域。虽然 HR 和 HP 的 SSU 组分的 pI 相同，但与 MF 的 SSU 不同，这表明 Medicago 的 SSU 存在异质性。有人认为，SSU 的碱性可能具有一些适应生理的意义。