花粉过氧化物酶同工酶的遗传学：10. prxD 基因的定位。

Genetics of the peroxidase isoenzymes inPetunia : 10. Location of the geneprxD.

机构信息

Institute of Genetics, University of Amsterdam, Kruislaan 318, NL-1098, SM Amsterdam, The Netherlands.

出版信息

Theor Appl Genet. 1985 Jul;69(4):349-51. doi: 10.1007/BF00570899.

Abstract

The structural geneprxD inPetunia codes for a slow moving anodic peroxidase whose activity is sensitive to high concentrations of hydrogen peroxide. The PRXd enzyme could be found in mature and old leaf and stem tissue of full-grown flowering plants. PRXd was found to be absent in tissues from flower corolla and root. The geneprxD is the fourth gene that codes for peroxidases in leaf and stem. Two mobility variants of the PRXd enzyme have been found among our inbred lines using starch gel system II electrophoresis. The geneprxD could be located on chromosome III by a four-point-cross involving the genesprxA, prxD, Mf1 andHt1. The order of the genes established is:Ht1 - Mf1 - prxD - prxA.

摘要

石竹的结构基因 PRXd 编码一种缓慢移动的阳极过氧化物酶，其活性对高浓度的过氧化氢敏感。PRXd 酶可以在成熟和老化的叶片和茎组织以及完全生长的开花植物的茎组织中找到。在花的花瓣和根组织中未发现 PRXd。geneprxD 是叶片和茎中编码过氧化物酶的第四个基因。在使用淀粉凝胶系统 II 电泳的自交系中，发现了 PRXd 酶的两种迁移变体。通过涉及基因 sprxA、prxD、Mf1 和 Ht1 的四点杂交，可以将 geneprxD 定位在染色体 III 上。确定的基因顺序为：Ht1 - Mf1 - prxD - sprxA。