Division of Molecular Plant Biology, University of California, 94720, Berkeley, CA, USA.
Planta. 1984 Nov;161(6):481-6. doi: 10.1007/BF00407079.
The appearance of photosynthetic proteins was directly measured in developing maize (Zea mays L.) leaves. Third leaves of 10-14-d-old seedlings were dissected into six successive sections from the basal meristem to the tip of the leaf. The membrane and soluble proteins were separated by polyacrylamide gel electrophoresis and then transferred onto cyanogen bromide paper. After transfer of membrane proteins the paper was reacted with antisera raised against the light-harvesting chlorophyll a/b protein of photosystem II, the chlorophyll a-binding protein of reaction center P-700 of photosystem I and the α-subunit of chloroplast-coupling factor 1. The blots of soluble proteins were reacted with antisera raised against the electron-transport proteins plastocyanin and Fe-NADP reductase (EC 1.6.7.1), the carbon-fixing enzymes ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) and phosphoenolpyruvate carboxylase (EC 4.1.1.31), as well as pyruvate orthophosphate dikinase (EC 2.7.9.1). The schedule of appearance of proteins shows that the light-harvesting and ATP-generating proteins are present in the most immature segments at the leaf base and accumulate rapidly as the cells mature. The carbon-reducing enzymes, however, appear only in tissue that has differentiated into mesophyll and bundle-sheath cells.
光合蛋白的出现直接测量了发育中的玉米（Zea mays L.）叶片。10-14 天龄幼苗的第三片叶子从基分生组织到叶片尖端被分成六个连续的部分。膜蛋白和可溶性蛋白通过聚丙烯酰胺凝胶电泳分离，然后转移到氰化溴化纤维素纸上。转移膜蛋白后，将纸与针对光系统 II 的捕光叶绿素 a/b 蛋白、光系统 I 的反应中心 P-700 的叶绿素 a 结合蛋白和叶绿体偶联因子 1 的α亚基的抗血清反应。可溶性蛋白的斑点与针对质体蓝素和 Fe-NADP 还原酶（EC 1.6.7.1）、固定碳酶核酮糖 1,5-双磷酸羧化酶（EC 4.1.1.39）和磷酸烯醇丙酮酸羧化酶（EC 4.1.1.31）以及丙酮酸正磷酸二激酶（EC 2.7.9.1）的抗血清反应。蛋白质出现的时间表表明，在叶基部最不成熟的部分存在着光收集和 ATP 生成蛋白，并且随着细胞的成熟而迅速积累。然而，碳还原酶仅出现在已经分化为叶肉和束鞘细胞的组织中。
