估计数量性状上单个基因的效应 1. 应用于 D. melanogaster 中 Est 6 的双列杂交法。

Estimating single gene effects on quantitative traits 1. A diallel method applied to Est 6 in D. melanogaster.

机构信息

Department of Biophysics and Theoretical Biology, The University of Chicago, 920 East 58th Street, 60627, Chicago, Ill, USA.

出版信息

Theor Appl Genet. 1985 Mar;69(5-6):625-9. doi: 10.1007/BF00251114.

DOI:10.1007/BF00251114

PMID:24254023

Abstract

A modified diallel cross is used to estimate effects of alleles at the esterase 6 locus, relative to strain and environmental variance, in Drosophila melanogaster. Three strains homozygous for Est 6 (s) and three homozygous for Est 6 (F) were crossed in all 36 combinations. Male progeny were scored for mating speed, copula duration and esterase 6 enzyme activity, and all progeny for developmental time. These alleles show a significant additive effect on mating speed, but not on the other traits. Copula duration, developmental time and enzyme activity show additive strain genetic variance. Enzyme activity and developmental time also have maternal or X-chromosome strain variance, and these two traits are significantly correlated. This modified diallel method is generally useful because it permits the partition of trait variance into additive and dominant locus, background genetic and environmental components.

摘要

采用改良的完全双列杂交方法，估算在黑腹果蝇中酯酶 6 基因座等位基因相对于品系和环境方差的效应。3 个 Est 6（s）纯合子品系和 3 个 Est 6（F）纯合子品系进行了 36 种组合的杂交。雄性后代的交配速度、交配持续时间和酯酶 6 酶活性以及所有后代的发育时间进行了评分。这些等位基因对交配速度有显著的加性效应，但对其他性状没有影响。交配持续时间、发育时间和酶活性显示出加性的品系遗传方差。酶活性和发育时间也具有母本或 X 染色体的品系方差，这两个性状显著相关。这种改良的完全双列杂交方法通常是有用的，因为它可以将性状方差分解为加性和显性基因座、背景遗传和环境成分。