铜离子对兔红细胞谷胱甘肽及溶血的影响。

The effect of copper ion on glutathione and hemolysis in rabbit erythrocytes.

机构信息

Dept. of Chemistry, Florida State University, 32306-3006, Tallahassee, FL.

出版信息

Biol Trace Elem Res. 1986 Dec;11(1):19-26. doi: 10.1007/BF02795519.

Abstract

The rate of hemolysis and the decline in glutathione (GSH) in rabbit erythrocytes caused by copper (Cu) ions were determined. Prior investigations have proposed that the oxidative stress induced by Cu ion depleted the normal cell protective mechanisms. The decline in GSH has been proposed as a necessary prerequisite for hemolysis. We have observed that both GSH decline and hemolysis are Cu dependent, but are two concurrent and independent processes. We have confirmed that oxygen is a necessary reactant for hemolysis and responsible for a major portion of GSH decline. However, in the presence of Cu ion, a slow decline in GSH occurs even in a deaerated system.

摘要

测定了铜 (Cu) 离子引起兔红细胞溶血和谷胱甘肽 (GSH) 下降的速率。先前的研究表明，Cu 离子诱导的氧化应激耗尽了正常的细胞保护机制。GSH 的下降已被提出是溶血的必要前提。我们观察到 GSH 的下降和溶血都是 Cu 依赖性的，但这是两个并发和独立的过程。我们已经证实，氧气是溶血所必需的反应物，并导致 GSH 下降的主要部分。然而，在 Cu 离子存在的情况下，即使在脱氧系统中，GSH 也会缓慢下降。