Dept. of Chemistry, Rutgers, The State University of New Jersey, New Brunswick, 08903, USA.
Biol Trace Elem Res. 1987 Apr;12(1):323-34. doi: 10.1007/BF02796689.
An external 3.8-MeV proton beam was employed to induce X-rays in 100-mg pellets of human follicular fluids and in 4-8 mg pellets of spermatozoa. The elements Cl, K, Ca, Fe, Cu, Zn, and Br were quantitatively determined in follicular fluids, whereas the elements, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, and Zn were determined in the spermatozoa. Both samples had high interelement Spearman correlation coefficients. Correlations of elements in the samples were observed with several clinical parameters. The multielemental analysis of spermatozoa can be used to approximate quantities of trace elements inserted into the egg ooplasm at the time of fertilization. These elemental quantities appear to be in the femtogram (10(-15)) range.
采用 3.8MeV 的外部质子束在 100mg 人滤泡液丸和 4-8mg 精子丸中诱发 X 射线。定量测定了滤泡液中的 Cl、K、Ca、Fe、Cu、Zn 和 Br 元素,而 S、Cl、K、Ca、Ti、V、Cr、Mn、Fe、Ni、Cu 和 Zn 元素则测定了精子中。两个样品的元素之间的 Spearman 相关系数都很高。观察到样品中元素的相关性与几个临床参数有关。精子的多元素分析可用于近似在受精时插入卵质的微量元素的数量。这些元素的数量似乎在飞克(10(-15))范围内。