U.S. Plant, Soil, and Nutrition Laboratory, USDA-ARS, Tower Road, 14853, Ithaca, New York.
J Chem Ecol. 1992 Oct;18(10):1727-41. doi: 10.1007/BF02751098.
Production of the mitochondrial ATPase inhibitory peptides efrapeptins was evaluated by HPLC analysis in 44 strains of nine species of the fungal genusTolypocladium (Deuteromycotina: Hyphomycetes). Efrapeptin identification was confirmed by mass spectral data for the first time in the speciesT. cylindrosporum. HPLC retention time data indicated thatT. nubicola andT. tundrense, two species not previously known to produce efrapeptins, also produce the peptides. No efrapeptins were detected (<0.3 mg/100 ml broth) in single strains each ofT. balanoides, T. extinguens, T. parasiticum, andT. microsporum. Five strains ofT. geodes produced detectable amounts of efrapeptins and had compound F, or F and G, as the major component(s) in the mixture with the order of abundance being F> or ∼G > H ∼ D ∼ E > C. Eleven strains ofT. niveum produced detectable amounts of efrapeptins and had efrapeptins D and E as the primary and secondary components, respectively, in the mixture with the order of abundance being D > E > F > C ∼ G. A singleT. niveum strain had an efrapeptin profile similar to that of theT. geodes strains. Ten strains ofT. cylindrosporum had detectable amounts of efrapeptins. Of these, nine had F and one had G as the major component.T. cylindrosporum had higher ratios of E to D than didT. geodes. Efrapeptins were detected in one of twoT. nubicola strains analyzed (F > G > H) and one of threeT. tundrense strains (F > G > H > E).T. niveum strains could, in most cases, be identified to species on the basis of their efrapeptin profiles.
采用高效液相色谱法（HPLC）分析了 9 个拟青霉属（Deuteromycotina：Hyphomycetes）真菌的 44 个菌株中，线粒体 ATP 酶抑制肽 efrapeptin 的产生情况。首次通过质荷比（mass spectral data）数据确认了 efrapeptin 在拟青霉属的一个种T. cylindrosporum 中存在。HPLC 保留时间数据表明，以前未知产生 efrapeptin 的两种真菌T. nubicola 和T. tundrense 也能产生该肽。在每个单菌株中，均未检测到 efrapeptin（<0.3 mg/100 ml 肉汤），这些菌株分别来自 T. balanoides、T. extinguens、T. parasiticum 和 T. microsporum。T. geodes 的 5 个菌株产生了可检测量的 efrapeptin，且混合物中的主要成分是化合物 F 或 F 和 G，其丰度顺序为 F>∼G>H∼D>E>C。T. niveum 的 11 个菌株产生了可检测量的 efrapeptin，混合物中的主要和次要成分分别为 efrapeptin D 和 E，其丰度顺序为 D>E>F>C∼G。T. niveum 的一个菌株具有与 T. geodes 菌株相似的 efrapeptin 图谱。T. cylindrosporum 的 10 个菌株产生了可检测量的 efrapeptin，其中 9 个菌株的主要成分为 F，一个菌株的主要成分为 G。与 T. geodes 相比，T. cylindrosporum 的 E 与 D 的比值更高。在分析的 2 个 T. nubicola 菌株中的 1 个菌株中（F>G>H）和 3 个 T. tundrense 菌株中的 1 个菌株中（F>G>H>E）均检测到 efrapeptin。在大多数情况下，T. niveum 菌株可根据其 efrapeptin 图谱鉴定到种。
