锐齿石栎的化感作用。

Allelopathy ofSasa cernua.

机构信息

Mizutani Plant Ecochemicals Project, ERATO, Research Development Corporation of Japan Eniwa Research Center, 3-1-1 Megumino-kita, 061-13, Eniwa, Japan.

出版信息

J Chem Ecol. 1992 Oct;18(10):1785-96. doi: 10.1007/BF02751103.

DOI:10.1007/BF02751103

PMID:24254720

Abstract

Sasa (Sasa cernua Makino) is a very serious weed pest. Its allelopathy was studied using lettuce, wheat, timothy, and green amaranth as testing species. Cultured in the rhizosphere soil ofSasa cernua, the seedlings were inhibited by 42-80% compared with the controls cultured in normal soil and vermiculite. The phenolic fraction extracted with 1 M NaOH from the rhizosphere soil ofS. cernua caused significant inhibitions on the seed germination and seedling growth of lettuce, timothy, green amaranth, and barnyard grass.p-Coumaric, ferulic, vanillic, andp-hydroxybenzoic acids andp-hydroxybenzaldehyde were identified as the main allelochemicals in sasa soil by HPLC and [(1)H]NMR. Their contents in the rhizosphere soil were 5640, 1060, 860, 810 and 630 μg/100 g soil. The neutral fraction inhibited the seed germination and seedling growth of lettuce in the TLC direct bioassay. Volatile compounds released from sasa leaves also inhibited the growth of lettuce, wheat, timothy, and green amaranth grown under light, and the growth of etiolated seedlings of barley and wheat. These results confirm thatS. cernua produces typical allelopathy through its rhizosphere soil and air space.

摘要

芒萁（Sasa cernua Makino）是一种非常严重的杂草害虫。本研究采用莴苣、小麦、梯牧草和绿苋菜作为供试物种，研究了其化感作用。与在正常土壤和蛭石中培养的对照相比，在芒萁根际土壤中培养的幼苗受到 42-80%的抑制。从芒萁根际土壤中用 1 M NaOH 提取的酚类物质对莴苣、梯牧草、绿苋菜和稗草的种子萌发和幼苗生长有显著抑制作用。通过 HPLC 和 [(1)H]NMR 鉴定出根际土壤中的主要化感物质为对香豆酸、阿魏酸、香草酸和对羟基苯甲酸以及对羟基苯甲醛。其含量分别为 5640、1060、860、810 和 630μg/100g 土壤。TLC 直接生物测定中，中性部分抑制莴苣种子的萌发和幼苗生长。从芒萁叶片释放的挥发性化合物也抑制了在光照下生长的莴苣、小麦、梯牧草和绿苋菜的生长，以及大麦和小麦黄化幼苗的生长。这些结果证实，芒萁通过其根际土壤和空气空间产生典型的化感作用。