Chemistry Division, Pakistan Institute of Nuclear Science and Technology, P. O. Nilore, Islamabad, Pakistan,
Biol Trace Elem Res. 2014 Jan;157(1):24-9. doi: 10.1007/s12011-013-9865-7. Epub 2013 Nov 20.
The study reports enhanced Fe, Cu, and Zn contents in breast tissues, a probable risk factor of breast cancer in females. Forty-one formalin-fixed breast tissues were analyzed using atomic absorption spectrophotometry. Twenty malignant, six adjacent to malignant and 15 benign tissues samples were investigated. The malignant tissues samples were of grade 11 and type invasive ductal carcinoma. The quantitative comparison between the elemental levels measured in the two types of specimen (benign and malignant) tissues (removed after surgery) suggests significant elevation of these metals (Fe, Cu, and Zn) in the malignant tissue. The specimens were collected just after mastectomy of women aged 19 to 59 years from the hospitals of Islamabad and Rawalpindi, Pakistan. Most of the patients belong to urban areas of Pakistan. Findings of study depict that these elements have a promising role in the initiation and development of carcinoma as consistent pattern of elevation for Fe, Cu, and Zn was observed. The results showed the excessive accumulation of Fe (229 ± 121 mg/L) in malignant breast tissue samples of patients (p < 0.05) to that in benign tissues samples (49.1 ± 11.4 mg/L). Findings indicated that excess accumulation of iron in malignant tissues can be a risk factor of breast cancer. In order to validate our method of analysis, certified reference material muscle tissue lyophilized (IAEA) MA-M-2/TM was analyzed for metal studied. Determined concentrations were quite in good agreement with certified levels. Asymmetric concentration distribution for Fe, Cu, and Zn was observed in both malignant and benign tissue samples.
研究报告称，乳腺癌女性乳房组织中的铁、铜和锌含量增加，这可能是乳腺癌的一个风险因素。采用原子吸收分光光度法分析了 41 例福尔马林固定的乳腺组织。研究了 20 例恶性、6 例恶性附近和 15 例良性组织样本。恶性组织样本为 11 级和浸润性导管癌型。对两种类型（良性和恶性）组织（手术后切除）中测量的元素水平进行定量比较，表明这些金属（铁、铜和锌）在恶性组织中显著升高。这些标本是从巴基斯坦伊斯兰堡和拉瓦尔品第的医院采集的，年龄在 19 岁至 59 岁之间的女性乳房切除术后。大多数患者属于巴基斯坦的城市地区。研究结果表明，这些元素在癌症的发生和发展中具有重要作用，因为观察到铁、铜和锌的升高模式一致。结果表明，患者恶性乳腺组织样本中铁（229 ± 121mg/L）的过度积累（p < 0.05）明显高于良性组织样本（49.1 ± 11.4mg/L）。研究结果表明，恶性组织中铁的过度积累可能是乳腺癌的一个风险因素。为了验证我们的分析方法，对铁、铜和锌研究的肌肉组织冻干（IAEA）MA-M-2/TM 认证参考材料进行了分析。测定的浓度与认证水平相当吻合。在恶性和良性组织样本中都观察到铁、铜和锌的不对称浓度分布。
