Lindberg S, Mercke U
Acta Otolaryngol. 1986 May-Jun;101(5-6):484-93. doi: 10.3109/00016488609108635.
The neuropeptide substance P (SP) is released from unmyelinated C-fibres in sensory nerves by antidromic nerve stimulation. In order to study the effect on mucociliary (m.c.) activity, the isolated distal end of the maxillary nerve was stimulated electrically (8 V, pulse rate 10 Hz, 5 ms pulses) 2-4 min, while the m.c. wave frequency in the maxillary sinus was recorded by a photoelectric technique. The m.c. wave frequency increased during stimulation by 35.4 +/- 4.9% (range 22.0-62.2%). Following pretreatment with atropine the m.c. activity increased by only 20.0 +/- 1.3% (range 14.9-21.9%) indicating that part of the response was mediated via stimulation of muscarinic receptors. The cholinergic part of the response is presumably secondary to release of SP since the SP antagonist [D-Pro2, D-Trp7,9]SP abolished the effect of nerve stimulation. Sympathetic ganglionectomy did not influence the response to antidromic nerve stimulation. It is concluded that antidromic stimulation of the maxillary nerve accelerates m.c. activity through a dual mechanism, involving release of SP, or SP-like peptides, from sensory C-fibre nerve endings as well as release of acetylcholine.
神经肽P物质（SP）通过逆向神经刺激从感觉神经的无髓鞘C纤维中释放出来。为了研究其对黏液纤毛（m.c.）活性的影响，对上颌神经的离体远端进行2 - 4分钟的电刺激（8伏，脉冲频率10赫兹，5毫秒脉冲），同时用光电技术记录上颌窦中的m.c.波频率。刺激期间，m.c.波频率增加了35.4 +/- 4.9%（范围为22.0 - 62.2%）。用阿托品预处理后，m.c.活性仅增加了20.0 +/- 1.3%（范围为14.9 - 21.9%），这表明部分反应是通过毒蕈碱受体的刺激介导的。反应的胆碱能部分可能继发于SP的释放，因为SP拮抗剂[D - Pro2，D - Trp7,9]SP消除了神经刺激的作用。交感神经节切除术不影响对逆向神经刺激的反应。结论是，上颌神经的逆向刺激通过双重机制加速m.c.活性，这涉及从感觉C纤维神经末梢释放SP或SP样肽以及乙酰胆碱的释放。
