Kayhan Seda, Sarı Nurşen, Nartop Dilek
Department of Chemistry, Faculty of Science, Gazi University , Ankara , Türkiye.
Artif Cells Nanomed Biotechnol. 2015;43(4):224-9. doi: 10.3109/21691401.2013.856014. Epub 2013 Nov 21.
We report a easy approach for the immobilization of glucose oxidase enzyme (GOx) on nanoparticles. Nanoparticles-modified polystyrene-ANH2 (PSA) with some salicylaldehyde derivatives were synthesized by means of condensation and investigated the enzymatic properties of GOx immobilized on there. Modified polystyrenes were characterized using IR spectra, gel permeation chromatography, and scanning electron microscopy. All the characteristics of the immobilized glucose oxidase (PSA-SalH)-GOx, (PSA-SalCH3)-GOx, and (PSA)-GOx enzyme showed except one of them. Immobilized GOx on to (PSA) showed two optimum pH, other immobilized polymers were one optimum.
我们报道了一种将葡萄糖氧化酶（GOx）固定在纳米颗粒上的简便方法。通过缩合反应合成了带有一些水杨醛衍生物的纳米颗粒修饰的聚苯乙烯-氨基（PSA），并研究了固定在其上的GOx的酶学性质。使用红外光谱、凝胶渗透色谱和扫描电子显微镜对修饰后的聚苯乙烯进行了表征。固定化葡萄糖氧化酶（PSA-SalH）-GOx、（PSA-SalCH3）-GOx和（PSA）-GOx酶的所有特性都表现出来了，只有其中一种除外。固定在（PSA）上的GOx显示出两个最佳pH值，而其他固定化聚合物只有一个最佳pH值。
