Sohn Won, Lee Oh Young, Lee Sang Pyo, Lee Kang Nyeong, Jun Dae Won, Lee Hang Lak, Yoon Byung Chul, Choi Ho Soon, Sim Jongmin, Jang Ki-Seok
Department of Internal Medicine, Hanyang University College of Medicine , Seoul , Korea.
Scand J Gastroenterol. 2014 Jan;49(1):43-51. doi: 10.3109/00365521.2013.857712. Epub 2013 Nov 21.
Recent studies have shown that mast cells play an important role in irritable bowel syndrome (IBS). We investigated the relationship between mast cells and the gut hormones substance P and vasoactive intestinal peptide (VIP) in irritable bowel syndrome with diarrhea (IBS-D).
Colonoscopic biopsies were performed on the rectal mucosa of 43 subjects (IBS-D patients: 22, healthy volunteers: 21) diagnosed according to the Rome III criteria. Mast cells, and substance P & VIP were evaluated by quantitative immunohistology and image analysis. Mast cells were counted as tryptase-positive cells in the lamina propria, and substance P and VIP levels were expressed as percentages of total areas of staining.
Mast cell counts were higher in IBS-D patients than healthy volunteers (9.6 ± 3.3 vs. 5.7 ± 2.5/high power field (HPF), p < 0.01). Substance P was also elevated (0.11 ± 0.08% vs. 0.03 ± 0.02 %, p < 0.01) while VIP was only high in women with IBS-D. Mast cell counts were positively correlated with levels of substance P & VIP in women but not men (women: r = 0.625, p < 0.01 for substance P and r = 0.651, p < 0.01 for VIP). However, mast cell counts were not correlated with IBS symptoms including abdominal pain.
Mast cells are activated leading to the raised levels of substance P & VIP in IBS-D patients. However, the correlation between mast cells and levels of substance P & VIP differs according to gender.
近期研究表明，肥大细胞在肠易激综合征（IBS）中起重要作用。我们调查了腹泻型肠易激综合征（IBS-D）患者中肥大细胞与肠道激素P物质和血管活性肠肽（VIP）之间的关系。
对43名根据罗马III标准诊断的受试者（IBS-D患者：22名，健康志愿者：21名）的直肠黏膜进行结肠镜活检。通过定量免疫组织化学和图像分析评估肥大细胞、P物质和VIP。肥大细胞计为固有层中色氨酸酶阳性细胞，P物质和VIP水平以染色总面积的百分比表示。
IBS-D患者的肥大细胞计数高于健康志愿者（9.6±3.3对5.7±2.5/高倍视野（HPF），p<0.01）。P物质也升高（0.11±0.08%对0.03±0.02%，p<0.01），而VIP仅在IBS-D女性中升高。女性肥大细胞计数与P物质和VIP水平呈正相关，男性则不然（女性：P物质r = 0.625，p<0.01；VIP r = 0.651，p<0.01）。然而，肥大细胞计数与包括腹痛在内的IBS症状无关。
IBS-D患者中肥大细胞被激活，导致P物质和VIP水平升高。然而，肥大细胞与P物质和VIP水平之间的相关性因性别而异。
