*Biology Department, Boston College, Chestnut Hill, MA 02467, U.S.A.
Biochem Soc Trans. 2013 Dec;41(6):1692-5. doi: 10.1042/BST20130159.
PKA (protein kinase A) in the fission yeast Schizosaccharomyces pombe controls transcription of genes involved in metabolism, cell growth and sexual development. In the present review, we discuss phenotypes associated with either high or low PKA activity in the context of how they can be used to carry out genetic or small-molecule screens that affect components of the PKA pathway. Although our recent research has focused on the study of heterologously expressed cyclic nucleotide PDEs (phosphodiesterases), these same methods can be used to target other S. pombe proteins or their functionally equivalent orthologues that act in the PKA pathway.
蛋白激酶 A（PKA）在裂殖酵母 Schizosaccharomyces pombe 中控制参与代谢、细胞生长和性发育的基因的转录。在本综述中，我们讨论了 PKA 活性高或低的表型，以及如何利用它们进行遗传或小分子筛选，从而影响 PKA 途径的组成部分。虽然我们最近的研究集中在研究异源表达的环核苷酸 PDEs（磷酸二酯酶）上，但这些相同的方法也可以用于靶向其他 S. pombe 蛋白或其在 PKA 途径中具有同等功能的同源物。
