University of Ljubljana, Biotechnical faculty , Department of Agronomy, Chair for Fruit, Wine and Vegetable Growing, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
J Agric Food Chem. 2013 Dec 11;61(49):12066-71. doi: 10.1021/jf404087f. Epub 2013 Nov 22.
Bitter pit is an important Ca(2+) deficiency disorder of apple fruit (Malus domestica Borkh.), with symptoms, necrotic spots, developing during storage. The objective of this study was to determine phenolic compounds and their contents in bitter pit in comparison to healthy skin and pulp using HPLC-MS(2). The experiment was carried out on three cultivars 'Jonagored', 'Golden Delicious' and 'Pinova'. All 15 determined phenolic compounds in pulp tissues specifically affected by bitter pit were higher than those in healthy pulp. Chlorogenic acid and catechin were to 5 times higher in those affected pulp tissues. Higher content was also determined for hydroxycinnamic acids and flavanols in the peel above the bitter pit; in contrast, flavonols and anthocyanins were higher in healthy peel. Anthocyanins in healthy peel of cultivar 'Jonagored' were 10 times higher from the content in peel above the bitter pit.
苦痘病是苹果果实（Malus domestica Borkh.）中一种重要的钙缺乏症，其症状为在贮藏过程中出现坏死斑点。本研究的目的是使用 HPLC-MS(2) 法测定苦痘病与健康果皮和果肉中的酚类化合物及其含量。该实验在三个品种“乔纳金”、“金冠”和“Pinova”上进行。果肉组织中所有 15 种受苦痘病影响的酚类化合物均高于健康果肉。受影响果肉组织中的绿原酸和儿茶素含量高出 5 倍。在苦痘上方的果皮中还检测到较高含量的羟基肉桂酸和黄烷醇，而在健康果皮中则检测到较高含量的类黄酮和花青素。品种“乔纳金”健康果皮中的花青素含量比苦痘上方果皮中的含量高出 10 倍。
