Chromosomal assignment of genes coding for the wheat gliadin protein components of the cultivars 'Cheyenne' and 'Chinese Spring' by two-dimensional (two-pH) electrophoresis.

The gliadin proteins of the hexaploid bread wheat cultivar 'Cheyenne' (Triticum aestivum L. var. aestivum), which has good mixing and baking characteristics, were analyzed by 2-dimensional (2-pH) polyacrylamide gel electrophoresis (pH 3.2 in the 1st dimension, pH 9.2 in the 2nd). Genes for most of the 35 resolved components were assigned to the chromosomes of homoeologous groups 1 and 6 through the use of various aneuploids and substitution lines. A similar analysis was carried out for the cultivar 'Chinese Spring', which does not have good quality, and which had been analyzed by a different 2-dimensional procedure by Wrigley and Shepherd (Ann NY Acad Sci 209:154, 1973). A coordinate system was devised for description of the components in the 2-dimensional patterns.