Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK.
Plant Cell Rep. 1983 Feb;2(1):55-7. doi: 10.1007/BF00269237.
Mesophyll protoplasts obtained from leaves of shoot cultures of Rehmannia glutinosa were cultured in Murashige and Skoog (1962) liquid or liquid-over-agar medium containing 2.0 mg L(-1) naphthaleneacetic acid and 0.5 mg L(-1) benzylamino purine. An amino acid mixture of glutamine, arginine, glycine, and aspartic acid promoted sustained protoplast division, with an average plating efficiency of 27%. Protoplast-derived colonies formed callus which readily regenerated shoots on fransfer to Murashige and Skoog based agar medium with 2.0 mg L(-1) indoleacetic acid and 1.0 mg L(-1) benzylamino purine. Leaf explants also showed a marked capacity for shoot regeneration in culture.
从地黄 shoot 培养物的叶片中获得的叶肉原生质体在 Murashige 和 Skoog（1962）液体或液体上琼脂培养基中培养，该培养基含有 2.0 mg L（-1）萘乙酸和 0.5 mg L（-1）苯氨基嘌呤。谷氨酸盐、精氨酸、甘氨酸和天冬氨酸的氨基酸混合物促进了原生质体的持续分裂，平均平板效率为 27%。原生质体衍生的菌落形成愈伤组织，易于在 Murashige 和 Skoog 基础琼脂培养基上再生芽，该培养基含有 2.0 mg L（-1）吲哚乙酸和 1.0 mg L（-1）苯氨基嘌呤。叶片外植体在培养中也表现出明显的芽再生能力。
