从地黄叶片原生质体和叶片外植体中进行 shoot 再生。

Shoot regeneration from mesophyll protoplasts and leaf explants of Rehmannia glutinosa.

机构信息

Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK.

出版信息

Plant Cell Rep. 1983 Feb;2(1):55-7. doi: 10.1007/BF00269237.

DOI:10.1007/BF00269237

PMID:24257858

Abstract

Mesophyll protoplasts obtained from leaves of shoot cultures of Rehmannia glutinosa were cultured in Murashige and Skoog (1962) liquid or liquid-over-agar medium containing 2.0 mg L(-1) naphthaleneacetic acid and 0.5 mg L(-1) benzylamino purine. An amino acid mixture of glutamine, arginine, glycine, and aspartic acid promoted sustained protoplast division, with an average plating efficiency of 27%. Protoplast-derived colonies formed callus which readily regenerated shoots on fransfer to Murashige and Skoog based agar medium with 2.0 mg L(-1) indoleacetic acid and 1.0 mg L(-1) benzylamino purine. Leaf explants also showed a marked capacity for shoot regeneration in culture.

摘要

从地黄 shoot 培养物的叶片中获得的叶肉原生质体在 Murashige 和 Skoog（1962）液体或液体上琼脂培养基中培养，该培养基含有 2.0 mg L（-1）萘乙酸和 0.5 mg L（-1）苯氨基嘌呤。谷氨酸盐、精氨酸、甘氨酸和天冬氨酸的氨基酸混合物促进了原生质体的持续分裂，平均平板效率为 27%。原生质体衍生的菌落形成愈伤组织，易于在 Murashige 和 Skoog 基础琼脂培养基上再生芽，该培养基含有 2.0 mg L（-1）吲哚乙酸和 1.0 mg L（-1）苯氨基嘌呤。叶片外植体在培养中也表现出明显的芽再生能力。