Institut für Biologie II, Universität Freiburg, Schänzlestr. 1, D-7800, Freiburg, FRG.
Plant Cell Rep. 1983 Apr;2(2):88-91. doi: 10.1007/BF00270173.
Transfer from complete to 2,4-D free Gamborg's B5-medium efficiently induced somatic embryogenesis in Papaver tissue cultures (P. somniferum and P. orientale). Embryogenesis was preceded by a strong temporary accumulation of triacylglycerols. In both tissue cultures large amounts of sanguinarine type alkaloids were present, which disappeared during regeneration in the P. orientale cultures but persisted in the P. somniferum cultures. In the P. somniferum cultures protopine and morphine type alkaloids (morphine, codeine, thebaine) appeared about 45 days after exchanging the medium. Thebaine was the main alkaloid in the P. somniferum embryoids accumulating up to 0.2 % of dry weight.
从完全培养基到 2,4-D 自由 Gamborg's B5 培养基的有效转化，可在罂粟组织培养物（罂粟和东方罂粟）中高效诱导体细胞胚胎发生。胚胎发生之前，三酰基甘油会大量暂时积累。在这两种组织培养物中，都存在大量血根碱型生物碱，这些生物碱在东方罂粟培养物的再生过程中消失，但在罂粟培养物中仍然存在。在罂粟培养物中，大约在更换培养基 45 天后，会出现原阿片碱和吗啡型生物碱（吗啡、可待因、蒂巴因）。蒂巴因是在积累高达 0.2%干重的罂粟胚状体中的主要生物碱。
