抑制与大豆叶绿体相关的脱氧核糖核酸酶活性。
Inhibition of deoxyribonuclease activity associated with soybean chloroplasts.
机构信息
Department of Genetics, Iowa State University, 50011, Ames, IA, USA.
出版信息
Plant Cell Rep. 1983 Apr;2(2):98-100. doi: 10.1007/BF00270176.
The effects of spermidine, pH, ethylene diamine tetracetic acid (EDTA), and adenosine triphosphate (ATP) on deoxyribonuclease (DNase) activity associated with the chloroplasts of soybean (Glycine max (L.) Merr.) were investigated. Chloroplast DNase activity was found to be partially inhibited by either 10 mM spermidine, 20 mM EDTA, or 20 mM ATP. DNase activity was also partially inhibited at non-neutral pH's. Nearly complete inhibition was achieved with use of 30 mM EDTA, pH 10, or a combination of 10 mM spermidine and 10 mM EDTA.
研究了腐胺、pH 值、乙二胺四乙酸（EDTA）和三磷酸腺苷（ATP）对大豆（Glycine max (L.) Merr.）叶绿体脱氧核糖核酸酶（DNase）活性的影响。发现腐胺 10mM、EDTA 20mM 或 ATP 20mM 可部分抑制叶绿体 DNase 活性。非中性 pH 值也会部分抑制 DNase 活性。使用 30mM EDTA、pH 值 10 或 10mM 腐胺和 10mM EDTA 的组合可实现几乎完全抑制。
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