叶片银荆花药培养产生单倍体植株

Production of haploid plantlets in anther cultures of Albizzia lebbeck L.

机构信息

Department of Botany, University of Delhi, 110007, Delhi, India.

出版信息

Plant Cell Rep. 1983 Dec;2(6):308-9. doi: 10.1007/BF00270188.

Abstract

Anthers of Albizzia lebbeck on B5 medium (BM) supplemented with kinetin (2 mg/l) and 2, 4-D (0.5 mg/l) showed callus initiation from microspores. Differentiation of embryoids and shoots was obtained on BM + BAP (1 mg/l) + IAA (0.5 mg/l) and of roots on BM. Root tip squashes of the regenerated plantlets showed the haploid chromosome number (n=13), confirming the microspore origin of the regenerants.

摘要

B5 培养基（BM）添加激动素（2 毫克/升）和 2,4-D（0.5 毫克/升）的厚荚相思花药可从小孢子诱导出愈伤组织。胚状体和芽的分化在 BM+BAP（1 毫克/升）+IAA（0.5 毫克/升）上获得，根在 BM 上获得。再生植株根尖压片显示出单倍体染色体数（n=13），证实了再生体的小孢子起源。