Institut für Allgemeine Botanik und Botanischer Garten, Ohnhorststrasse 18, D-2000, Hamburg 52, Federal Republic of Germany.
Planta. 1984 Mar;160(3):217-21. doi: 10.1007/BF00402857.
Prostrate cells of Micrasterias thomasiana Archer were irradiated from above with intensive blue light. Many of the cells reacted by rising to a profile position. During a period of 15 to 90 min the response is linearly dependent on the duration of irradiation, inferred from the number of rising cells. In a range from 10 to 30 μmol m(-2) s(-1) (equalling 2.7-8.0 W m(-2) at a wavelength of 450 nm) and for an irradiation time of 30 min, the rising reaction was linearly dependent on the quantum flux density. Choosing 30 min irradiation time and a quantum flux density of 30 μmol m(-2) s(-1), the reactive number of rising cells was employed in establishing an action spectrum. As a result of this, a flavin is postulated as the light-percepting pigment in the reaction, whereas chlorophylls do not appear to be involved. The rising reaction can be distinguished from other light-induced movements as a strong-light response, resembling in this respect the movement of chloroplasts within cells. The different sensitivity of individual cells and the importance of this strong-light response for the algal cells is discussed.
用高强度蓝光从上方照射小环藻的前列腺细胞，许多细胞通过上升到轮廓位置来做出反应。在 15 到 90 分钟的时间内，反应与辐照时间呈线性相关，这可以从上升细胞的数量推断出来。在 10 到 30 μmol m(-2) s(-1)（在 450nm 波长处相当于 2.7-8.0 W m(-2)）和 30 分钟的辐照时间范围内，上升反应与量子通量密度呈线性相关。选择 30 分钟的辐照时间和 30 μmol m(-2) s(-1)的量子通量密度，上升反应的活性细胞数量用于建立作用光谱。因此，假定黄素是反应中的光感受色素，而叶绿素似乎不参与其中。上升反应可以与其他光诱导的运动区分开来，因为它是一种强光反应，在这方面类似于细胞内叶绿体的运动。讨论了个别细胞的不同敏感性以及这种强光反应对藻类细胞的重要性。
