Research Department, Nestlé Products Technical Assistance CO., CH-1814, La Tour-de-Peilz, Switzerland.
Planta. 1984 Mar;160(4):314-9. doi: 10.1007/BF00393412.
Hypocotyls of 5-d-old etiolated soybean seedlings (Glycine max (L.) Merr. cv. Altona) were treated with (a) dithiothreitol (DTT) or one of the sulfhydryl-binding reagents N-ethylmaleimide (NEM), p-hydroxymercuribenzoate (PMB) und p-chloromercuribenzene sulfonic acid (PMBS), (b) one of the sulfhydryl reagents in combination with DTT, (c) sulfhydryl reagent subsequent to treatment with DTT, and (d) PMBS followed by DTT. Glyceollin was extracted 24 and 48 h after initiation of treatment. The order of decreasing glyceollin-eliciting activity was PMBS≫DTT>PMB≧NEM. Elicitor effectiveness of sulfhydryl reagents and their reactivity with either L-cysteine or sulfhydryl groups in soybean hypocotyls were not strictly correlated. Mixtures of sulfhydryl reagent and DTT, pretreatment of hypocotyls with DTT and subsequent application of either PMB or PMBS, as well as application of PMBS prior to DTT induced less glyceollin than sulfhydryl reagents alone. In contrast, such pretreatment did not appreciably alter glyceollin accumulation elicited by NEM. The results indicate that glyceollin synthesis can be regulated by interaction with sulfhydryl groups located mainly at the outer surface of the plasmalemma.
5 天龄的黄化大豆幼苗(Glycine max (L.) Merr. cv. Altona)下胚轴用(a)二硫苏糖醇(DTT)或巯基结合试剂 N-乙基马来酰亚胺(NEM)、对羟汞苯甲酸(PMB)和对氯汞苯磺酸(PMBS)、(b)一种巯基试剂与 DTT 联合处理、(c)DTT 处理后用巯基试剂处理、和(d)PMBS 后用 DTT 处理。在处理开始后 24 和 48 小时提取大豆球蛋白。大豆球蛋白诱导活性降低的顺序为 PMBS≫DTT>PMB≧NEM。巯基试剂的诱导剂有效性及其与 L-半胱氨酸或大豆下胚轴中巯基的反应性没有严格相关。巯基试剂和 DTT 的混合物、用 DTT 预处理下胚轴以及随后应用 PMB 或 PMBS,以及在 DTT 之前应用 PMBS 诱导的大豆球蛋白比单独使用巯基试剂少。相比之下,这种预处理不会明显改变 NEM 引起的大豆球蛋白积累。结果表明,大豆球蛋白的合成可以通过与主要位于质膜外表面的巯基相互作用来调节。