藻质体己糖-质子协同转运系统转运蛋白中的必需巯基。
Essential Sulfhydryl Group in the Transport-catalyzing Protein of the Hexose-Proton Cotransport System of Chlorella.
机构信息
Botanik I, Fachbereich Biologie und Vorklinische Medizin Universität Regensburg, West Germany.
出版信息
Plant Physiol. 1978 May;61(5):785-6. doi: 10.1104/pp.61.5.785.
Abstract
The polyene antibiotic nystatin transforms the sugar-proton contransport system of Chlorella to a mere facilitated diffusion system. This experimental condition was used to test the sugar-translocating unit of the active uptake system for possible essential sulfhydryl groups. It could be shown that the catalyzed translocation of sugar is sensitive to the sulfhydryl-reactive compound N-ethylmaleimide. Sugar flow by passive leak as induced by the detergent Triton X-100 is not affected by sulfhydryl reagents. These results show that the sugar-translocating carrier protein possesses a sulfhydryl group, which is essential for its function.
摘要
多烯抗生素制霉菌素将小球藻的糖-质子共运输系统转化为单纯的易化扩散系统。这种实验条件被用于测试主动摄取系统的糖转运单位是否存在可能的必需巯基。结果表明，糖的催化转运对巯基反应性化合物 N-乙基马来酰亚胺敏感。由去污剂 Triton X-100 诱导的被动渗漏引起的糖流不受巯基试剂的影响。这些结果表明，糖转运载体蛋白含有一个巯基，该巯基对于其功能是必需的。
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