微流控聚甲基丙烯酸甲酯芯片中镧系元素的免疫血小板减少症

ITP of lanthanides in microfluidic PMMA chip.

作者信息

Cong Yongzheng, Bottenus Danny, Liu Bingwen, Clark Sue B, Ivory Cornelius F

机构信息

Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA.

出版信息

Electrophoresis. 2014 Mar;35(5):646-53. doi: 10.1002/elps.201300382. Epub 2013 Dec 27.

DOI:10.1002/elps.201300382

PMID:24258617

Abstract

An ITP separation of eight lanthanides on a serpentine PMMA microchip with a tee junction and a 230-mm-long serpentine channel is described. The cover of the PMMA chip is 175 μm thick so that a C(4) D in microchip mode can be used to detect the lanthanides as they migrate through the microchannel. Acetate and α-hydroxyisobutyric acid are used as complexing agents to increase the electrophoretic mobility difference between the lanthanides. Eight lanthanides are concentrated within ∼ 6 min by ITP in the microchip using 10 mM ammonium acetate at pH 4.5 as the leading electrolyte and 10 mM acetic acid at ∼ pH 3.0 as the terminating electrolyte. In addition, a 2D numerical simulation of the lanthanides undergoing ITP in the microchip is compared with experimental results using COMSOL Multiphysics v4.3a.

摘要

描述了在具有三通连接和230毫米长蛇形通道的蛇形聚甲基丙烯酸甲酯（PMMA）微芯片上对八种镧系元素进行等速电泳（ITP）分离的方法。PMMA芯片的盖子厚度为175μm，因此在微芯片模式下可以使用C(4) D来检测镧系元素在微通道中迁移时的情况。醋酸盐和α-羟基异丁酸用作络合剂，以增加镧系元素之间的电泳迁移率差异。在微芯片中，以pH 4.5的10 mM醋酸铵作为前导电解质，以约pH 3.0的10 mM醋酸作为终止电解质，通过ITP在约6分钟内将八种镧系元素浓缩。此外，使用COMSOL Multiphysics v4.3a将微芯片中镧系元素进行ITP的二维数值模拟与实验结果进行了比较。