USDA, ARS, MA VERL, 32604, Gainesville, Florida.
J Chem Ecol. 1991 Feb;17(2):267-84. doi: 10.1007/BF00994332.
The major alkene of the male tsetse fly,Glossina morsitans morsitans, was isolated for characterization by thin-layer and gas chromatography (GC). The mass spectra of the alkene and the alkene DMDS derivative indicated one isomer, 19,23-dimethyltritriacont-1-ene. The material is present at 1-2μg/male fly and is partially transferred to the female preparatory to or during mating. A dose-dependent antiaphrodisiac effect was seen with exposed male flies using the isolated natural product, with 2 and 4μg causing 80% loss of copulatory attempts, and 10μg extinguishing the attempts. This effect was increased by addition of male-produced alkane. This compound and a 31-carbon homolog also appear inG. m. submorsitans. Similar quantities of alkenes that are species-specific appear in all tsetse males. Structures of male-produced trimethylalkenes that appear in two other species,G. palpalis palpalis andG. fuscipes fuscipes, were investigated.
采采蝇雄性的主要烯烃，舌蝇 Glossina morsitans morsitans，通过薄层色谱和气相色谱（GC）进行分离和特性鉴定。烯烃和 DMDS 衍生物的质谱表明存在一种异构体，19,23-二甲基三十碳-1-烯。该物质在每只雄性采采蝇中存在 1-2μg，并在交配前或交配期间部分转移到雌性。使用分离的天然产物，暴露的雄性苍蝇表现出剂量依赖性的抗性欲作用，2μg 和 4μg 导致交配尝试的 80%丧失，而 10μg 则完全消除了交配尝试。添加雄性产生的烷烃会增加这种效果。这种化合物和 31 个碳原子的同系物也出现在舌蝇亚种 submorsitans 中。在所有雄性采采蝇中，也存在类似数量的具有种特异性的烯烃。还研究了另外两种采采蝇，G. palpalis palpalis 和 G. fuscipes fuscipes 中出现的雄性产生的三甲基烯烃的结构。
