小麦和水稻的冰冻花粉胚和受精卵胚胎的植物再生。
The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice.
机构信息
Tissue Culture Laboratory, Punjab Agricultural University, Ludhiana, India.
出版信息
Theor Appl Genet. 1984 Apr;67(6):525-8. doi: 10.1007/BF00264898.
PMID:24258841
Abstract
Anther culture derived pollen embryos and immature zygotic embryos of wheat and rice, frozen in liquid nitrogen in the presence of dimethyl sulfoxide, sucrose and glycerol, have been revived. The retrieved cultures proliferated and/or regenerated shoots and plantlets. The prospects of the cryopreservation of embryos for the conservation and multiplication of germplasm and the possibility of the establishment of 'Germplasm Banks' are discussed.
摘要
从小麦和水稻中提取的花粉胚胎和未成熟的合子胚胎，在二甲基亚砜、蔗糖和甘油的存在下，经过液氮冷冻，已经被复苏。回收的培养物增殖和/或再生出芽和小植株。本文讨论了胚胎冷冻保存对于种质资源的保存和繁殖的前景，以及建立“种质库”的可能性。
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Plant Physiol. 1979 Mar;63(3):460-7. doi: 10.1104/pp.63.3.460.
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