Natural Resources Institute, Central Avenue, Chatham Maritime, ME4 4TB, Chatham, Kent, England.
J Chem Ecol. 1991 Apr;17(4):789-803. doi: 10.1007/BF00994201.
Male beetles of the larger grain borer,Prostephanus truncatus Horn (Coleoptera: Bostrichidae) were shown to produce material causing an electroantennographic (EAG) response from both male and female beetles. Volatiles from mixed sex cultures were collected on Porapak Q and fractionated by liquid chromatography (LC) with gradient elution. The total volatiles and the fractions were analyzed by gas chromatography (GC) linked to EAG, and two compounds eluting in the same LC fraction elicited EAG responses from male and female beetles. These were assumed to be components of the male-produced aggregation pheromone. Amounts of these compounds obtained were very low and less than 10(-3) times the amounts of the aggregation pheromone components produced by the related species,Rhyzopertha dominica, under similar conditions. The most abundant of these EAG-active compounds was identified as 1-methyiethyl (2E)-2-methyl-2-pentenoate by comparison of its GC retention times and mass spectrum with those of synthetic analogs. The synthetic compound elicited electrophysiological and behavioral responses in both male and female beetles significantly greater than those to structural analogs. This compound is attractive toP. truncatus beetles in the field, and has been given the trivial name of "trunc-call 1".
雄性谷蠹（Prostephanus truncatus Horn）（鞘翅目：象甲科）会产生一种物质，这种物质能引起雌雄两性谷蠹触角电位（EAG）反应。雌雄混合培养物的挥发物用 Porapak Q 收集，并通过液相色谱（LC）进行梯度洗脱进行分离。用气相色谱（GC）连接 EAG 对总挥发物和馏分进行分析，在相同的 LC 馏分中洗脱的两种化合物能引起雌雄两性谷蠹的 EAG 反应。这些化合物被认为是雄性产生的聚集信息素的组成部分。这些化合物的含量非常低，不到在类似条件下产生的相关种——玉米象（Rhyzopertha dominica）聚集信息素成分的 10（-3）倍。通过比较其 GC 保留时间和质谱与合成类似物的结果，这些 EAG 活性化合物中最丰富的一种被鉴定为 1-甲基乙基（2E）-2-甲基-2-戊烯酸酯。该合成化合物在雌雄两性谷蠹中引起的电生理和行为反应明显大于结构类似物，该化合物对田间的谷蠹具有吸引力，并被赋予了“trunc-call 1”的俗名。
